关键词: 子宫内膜炎, 大肠埃希菌, 金黄色葡萄球菌, LPS, 小鼠动物模型

Abstract: Objective　To conduct a comparative study on mice models of endometritis induced by Staphylococcus aureus(S. aureus), Escherichia coli (E. coli), and lipopolysaccharide (LPS).Methods Twenty-four female ICR mice were randomly divided into four groups:the control group, the LPS model group, the S. aureus model group, and the E. coli model group, with 6 mice in each group. The inducing agents injected were normal saline, LPS (1 mg/mL), S. aureus (1×109 CFU/mL), and E. coli (1×108 CFU/mL) respectively, at a dose of 50 μL per mouse. The mice were weighed continuously for 7 days. On the 7th day, blood and uterine tissues were collected to detect blood routine and blood biochemical parameters, calculate the uterine index, observe the structure of uterine pathological tissue sections, and determine the changes in inflammatory factors TNF-α and IL-1β in uterine tissues.Results Compared with the control group, the mice in the two bacterial-induced model groups showed a significant increase in white blood cell count, mainly due to the elevation of neutrophils, with significant differences (P<0.05). The uterine index in the S. aureus group increased significantly (P<0.05); the histopathological sections of the three model groups showed varying degrees of endometrial cell damage, loss, and neutrophil exudation; the expression levels of inflammatory factors TNF-α and IL-1β in the S. aureus group and E. coli group increased significantly (P<0.01).Conclusion　This study successfully constructed three mice models of endometritis induced by different factors (S. aureus, E. coli, and LPS), and established the S. aureus-induced mouse endometritis model for the first time. By comparing the blood, tissue, and cytokine aspects, the differences among different induced models were revealed, providing an experimental model for further research on endometritis. 

Key words: endometritis, Escherichia coli, Staphylococcus aureus, LPS, murine animal model